Ryohei Yasuda, PhD

Ryohei Yasuda, PhD

Research Group Leader, Scientific Director
Neuronal Signal Transduction

One Max Planck Way
Jupiter, FL 33458
(561) 972-9000

Visit Ryohei Yasuda's Lab Website

Researcher Bio

Dr. Ryohei Yasuda was named Scientific Director of the Max Planck Florida Institute for Neuroscience in 2012 and heads the Neuronal Signal Transduction Lab. The focus of Dr. Yasuda’s lab is to elucidate some of the molecular mechanisms underlying synaptic plasticity and ultimately learning and memory.

Dr. Yasuda received his PhD in Physics in 1998 from Keio University Graduate School of Science and Technology in Yokohama, Japan. In his PhD studies, he demonstrated that the enzyme ATP synthase is a rotary motor made of single molecule and that its energy conversion efficiency is close to 100%. From 2000 to 2005, he was a post-doctoral fellow at the Cold Spring Harbor Laboratory where he built an imaging device to monitor protein interactions in living cells with high sensitivity and resolution. From 2005 to 2012, he was an assistant professor of the Neurobiology department at the Duke University Medical Center where he developed a number of techniques to visualize signaling activity in single synapses. From 2009 to 2012, Dr. Yasuda served as an Early Career Scientist at the Howard Hughes Medical Institute.

Dr. Yasuda has received a number of awards for his research accomplishments including the Career Award at the Scientific Interface from the Burroughs Wellcome Fund, the Alfred P. Sloan Fellowship, the New Investigator Award from the Alzheimer’s Association, and Research Award for Innovative Neuroscience from the Society for Neuroscience.

Selected Publications

  1. Colgan, L.A., Hu, M., Misler, J.A., Parra-Bueno, P., Moran, C.M., Leitges, M., and Yasuda, R. (2018). PKCα integrates spatiotemporally distinct Ca2+ and autocrine BDNF signaling to facilitate synaptic plasticity. Nature Neuroscience. Online Publication.

  2. Smirnov, M.S., Garret, T.R., and Yasuda, R. (2018). An open-source tool for analysis and automatic identification of dendritic spines using machine learning. PLOS ONE. Online Publication.

  3. Evans, P.R., Parra-Bueno, P., Smirnov, M.S., Lustberg, D.J., Dudek, S.M., Hepler, J.R., and Yasuda, R. (2018). RGS14 Restricts Plasticity in Hippocampal CA2 by Limiting Postsynaptic Calcium Signaling. ENeuro ENEURO.0353-17.2018.

  4. Yasuda, R. (2017). Biophysics of Biochemical Signaling in Dendritic Spines: Implications in Synaptic Plasticity. Biophysical Journal 113, 1-8.

  5. Jun Nishiyama, Takayasu Mikuni, and Ryohei Yasuda (2017). Virus-Mediated Genome Editing via Homology-Directed Repair in Mitotic and Postmitotic Cells in Mammalian Brain. Neuron, Advance Online Publication.

  6. Hedrick, N.G., Yasuda, R. (2017).  Regulation of Rho GTPase proteins during spine structural plasticity for the control of local dendritic plasticity. Curr. Opin. Neurobiol. 45, 193-201.

  7. Chang, J.-Y., Parra-Bueno, P., Laviv, T., Szatmari, E.M., Lee, S.-J.R., and Yasuda, R. (2017). CaMKII Autophosphorylation Is Necessary for Optimal Integration of Ca2+ Signals during LTP Induction, but Not Maintenance. Neuron 94, 800–808.e4.

  8. Murakoshi, H., Shin, M.E., Parra-Bueno, P., Szatmari, E.M., Shibata, A.C.E., Yasuda, R. (2017). Kinetics of Endogenous CaMKII Required for Synaptic Plasticity Revealed by Optogenetic Kinase Inhibitor. Neuron, Online Publication.

  9. Tang, S., and Yasuda, R. (2017). Imaging ERK and PKA Activation in Single Dendritic Spines during Structural Plasticity. Neuron, Online Publication.

  10. Smirnov, M.S., Evans, P.R., Garrett, T.R., Yan, L., Yasuda, R. (2017). Automated Remote Focusing, Drift Correction, and Photostimulation to Evaluate Structural Plasticity in Dendritic Spines. PLoS One 12, e0170586.

  11. Laviv, T., Kim, B.B., Chu, J., Lam, A.J., Lin, M.Z., and Yasuda, R. (2016). Simultaneous dual-color fluorescence lifetime imaging with novel red-shifted fluorescent proteins. Nature Methods 13, 989-992.

  12. Harward, S.C., Hedrick, N.G., Hall, C.E., Parra-Bueno, P., Milner, T.A., Pan, E., Laviv, T., Hempstead, B.L., Yasuda, R., and McNamara, J.O. (2016). Autocrine BDNF–TrkB signalling within a single dendritic spine. Nature 538, 99-103.

  13. Hedrick, N.G., Harward, S.C., Hall, C.E., Murakoshi, H., McNamara, J.O., and Yasuda, R. (2016). Rho GTPase complementation underlies BDNF-dependent homo- and heterosynaptic plasticity. Nature 538, 104-108.

  14. Chu, J., Oh, Y., Sens, A., Ataie, N., Dana, H., Macklin, J.J., Laviv, T., Welf, E.S., Dean, K.M., Zhang, F., et al. (2016). A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo. Nat. Biotechnol. 34, 760–767.

  15. Mikuni, T., Nishiyama, J., Sun, Y., Kamasawa, N., and Yasuda, R. (2016). High-Throughput, High-Resolution Mapping of Protein Localization in Mammalian Brain by In Vivo Genome Editing. Cell 165, 1803–1817.

  16. Rzuczek, S.G., Colgan, L.A., Nakai, Y., Cameron, M.D., Furling, D., Yasuda, R., and Disney, M.D. (2017).  Precise small-molecule recognition of a toxic CUG RNA repeat expansion.. Nat. Chem. Biol. 13, 188–193.

  17. Kamasawa, N., Sun, Ye., Mikuni, T., Guerrero-Given, D., Yasuda, R. (2015). Correlative Ultrastructural Analysis of Functionally Modulated Synapses Using Automatic Tape-Collecting Ultramicrotome – SEM Array Tomography. Microscopy and Microanalysis 21, 1271-1272.

  18. Nishiyama, J. and Yasuda, R. (2015). Biochemical Computation for Spine Structural Plasticity. Neuron. 87, 63-75.

  19. Otmakhov, N., Gorbacheva, E.V., Regmi, S., Yasuda, R., Hudmon, A., Lisman, J. (2015). Excitotoxic insult results in a long-lasting activation of CaMKIIα and mitochondrial damage in living hippocampal neurons. PLoS ONE 10, e0120881.

  20. Shibata, M., Uchihashi, T., Ando, T., Yasuda, R. (2015). Long-tip high-speed atomic force microscopy for nanometer-scale imaging in live cells. Scientific Reports 5, 8724.

  21. Kim, I.H., Wang, H., Soderling, S.H., Yasuda, R. (2014). Loss of Cdc42 leads to defects in synaptic plasticity and remote memory recall. Elife 3, e02839.

  22. Oliveira, A.F. and Yasuda, R. (2014). Neurofibromin is the major ras inactivator in dendritic spines. J Neurosci. 34, 776-83.

  23. Zhai, S., Ark, E.D.,  Parra-Bueno, P.,  Yasuda, R. (2013). Long-distance integration of nuclear ERK signaling triggered by activation of a few dendritic spines. Science. 342, 1107-11.

  24. Colgan, L.A., Yasuda,  R. (2013). Plasticity of Dendritic Spines: Subcompartmentalization of Signaling. Annu Rev Physiol. 76, 365-385.

  25. Oliveira, A.F. and Yasuda, R. (2014) Imaging the activity of Ras superfamily GTPase proteins in small subcellular compartments in neurons. Methods Mol Biol. 1071, 109-128.

  26. Szatmari, E. M., Oliveira, A.F., Sumner, E.J. and Yasuda R. (2013) Centaurin-α1-Ras-Elk-1 signaling at mitochondria mediates β-amyloid-induced synaptic dysfunction. J Neurosci. 33, 5367-5374.

  27. Watanabe, H., Uchihashi, T., Kobashi, T., Shibata, M., Nishiyama, J., Yasuda, R., Ando, T. (2013). Wide-area scanner for high-speed atomic force microscopy. Rev Sci Instrum. 84, 053702.

  28. Kim, I.H., Racz, B., Wang, H., Burianek, L., Weinberg, R., Yasuda R., Wetsel, W.C., Soderling, S.H. (2013) Disruption of Arp2/3 results in asymmetric structural plasticity of dendritic spines and progressive synaptic and behavioral abnormalities. J Neurosci. 33, 6081-92.

  29. Oliveira, A.F. and Yasuda, R. (2013) An improved Ras sensor for highly sensitive and quantitative FRET-FLIM imaging. PLoS One. 8, e52874.

  30. Uezu, A., Okada, H, Murakoshi, H., del Vescovo, C.D., Yasuda, R., Diviani, D., Soderling, S.T. (2012). Modified SH2 domain to phototrap and identify phosphotyrosine proteins from subcellular sites within cells.. Proc. Natl. Acad. Sci. U.S.A. 109, E2929-2938.

  31. Chen, Q., Cichon, J., Wang, W., Qiu, L., Lee, S.R., Campbell, N.R., Destefino, N., Goard, M.J., Fu, Z., Yasuda, R., Looger, L.L., Arenkiel, B.R., Gan, W., Feng, G. (2012). Imaging neural activity using Thy1-GCaMP transgenic mice.. Neuron 76, 297-308.

  32. Yasuda, R. (2012). Studying signal transduction in single dendritic spines. Cold Spring Harb Perspect Biol 04, 1-16.

  33. Lisman, J., R. Yasuda R., Raghavachari, S. (2012) Mechanisms of postsynaptic CaMKII action in synaptic memory. Nat. Rev. Neurosci. 13, 169-82.

  34. Murakoshi, H. and Yasuda, R. (2012)Postsynaptic signaling during plasticity of dendritic spines. Trends. Neurosci. 35, 135-43.

  35. Murakoshi, H., Wang, H., Yasuda,  R. (2011). Localized, persistent activation of Rho GTPases during long-term structural plasticity induced in single dendritic spines. Nature, 472, 100-4.

  36. Ramsey, A.J., Milenkovic, M., Oliveira, A.F., Escobedo-Lozoya, Y., Seshadri, S., Salahpour, A., Sawa, A., Yasuda, R., Caron, M.G. (2011). Impaired NMDA receptor transmission alters striatal synapses and DISC1 protein in an age-dependent manner.. Proc. Natl. Acad. Sci. U.S.A. 108, 5795-5800.

  37. Yasuda, R. and Murakoshi, H. (2011) The mechanisms underlying the spatial spreading of signaling activity. Curr. Opin. Neurobiol. 21, 313-21.

  38. Patterson, M., Yasuda, R. (2011). Signaling pathways underlying structural plasticity of dendritic spines. Br. J. Pharmacol. 163, 1626-38.

  39. Li, J., Kanju, P., Patterson, M., Chew, W., Cho, S., Gilmour, I., Oliver, T., Yasuda, R., Ghio, A., Simon, S.A., Liedtke, W. (2011). TRPV4-mediated calcium influx into human bronchial epithelia upon exposure to diesel exhaust particles. Environ. Health Perspect. 119, 784-793.

  40. M. A. Patterson, E. M. Szatmari, R. Yasuda. (2010) AMPA receptors are exocytosed in stimulated spines and adjacent dendrites in a Ras-ERK-dependent manner during long-term potentiation. Proc. Natl. Acad. Sci. U.S.A. 107, 15951-6.

  41. Lee, M. C., Yasuda, R., Ehlers, M. D. (2010) Metaplasticity at single glutamatergic synapses. Neuron 66, 859-70.

  42. Simmons, S. B., Escobedo, Y., Yasuda, R., Dudek, S. M. (2009) Regional differences in hippocampal calcium handling: a cellular mechanism for limiting plasticity. Proc. Natl. Acad. Sci. USA 106, 14080-4.

  43. S.-J. R. Lee, Y. Escobedo-Lozoya, E. M. Szatmari, R. Yasuda.(2009) Activation of CaMKII in single dendritic spines during long-term potentiation. Nature 458, 299-304.

  44. Lee, S.-J. R. and Yasuda, R. (2009) Spatiotemporal regulation of signaling in and out of dendritic spines: CaMKII and Ras. Open Neurosci. J. 3, 117-127.

  45. Murakoshi, H., Lee, S.-J. and Yasuda, R. (2008) Highly sensitive and quantitative FRET-FLIM imaging in single dendritic spines using improved non-radiative YFP. Brain Cell Biol. 36:31-42.

  46. Harvey*, C. D., Yasuda*, R., Zhong, H.  and Svoboda, K. (2008) The spread of Ras activity triggered by activation of a single dendritic spine. Science 321, 136-140.